A hybridization technique for orphan legumes: development of an artificial interspecific pollination protocol for Crotalaria spp.

Abstract

rotalaria brevidens: and C. ochroleuca commonly grown as vegetables have been extensively studied. However, information

on breeding techniques is scanty. This study aimed to develop an artificial interspecific pollination protocol for two genotypes of Crotalaria with contrasting leaf and pod morphological

features. The two are landraces that were previously

collected from two counties in Kenya. The experimental design was a completely randomized design (CRD) in a greenhouse with C. ochroleuca as the female parent and C. brevidens as the male parent. Six-day-old flower buds of the female parent were emasculated. Pollen from a freshly opened flower was rubbed over the stigma of the emasculated flower. The pollinated stigma was inserted back into the

keel petal and covered by the wing and standard petal. Data

on success rate, pod, and seed production were subjected to

analysis

of variance. The developed artificial pollination method showed 75% success rate in the interspecies cross of C. ochroleuca and C. brevidens. Time of crossing did not significantly influence the success rate, pod, and seed production. The study demonstrated artificial interspecific pollination

of Crotalaria

by keel petal incision rubbing method. This protocol

lays the foundation for genetic studies and improvement of Crotalaria spp.