Abstract:
Paenibacillus larvae is a spore-forming bacterium causing American foulbrood (AFB) in
honey bee larvae. The remains of a diseased larva contains billions of extremely resilient P.
larvae spores viable for decades. Burning clinically symptomatic colonies is widely
considered the only workable strategy to prevent further spread of the disease, and the
management practices used for decontamination requires high concentrations of chemicals or
special equipment.
The aim of this study was to test and compare the biocidal effect of two commercially
available disinfectants, “Disinfection for beekeeping” and Virkon S on P. larvae. The two
products were applied to P. larvae spores in suspension as well as inoculated on two common
beehive materials, wood and styrofoam.
“Disinfection for beekeeping” had a 100 % biocidal effect on P. larvae spores in suspension
compared to 87.0-88.6 % for Virkon S which, however, had a significantly better effect on P.
larvae on styrofoam. The two disinfectants had similar effect on infected wood material.
