Abstract:
Banana (Musa spp) is an important economic resource for rural farmers in Kenya. However,
bananas are being affected by several constraints such as insect pests and diseases. Some of the
pathogens have mutated and become resistant to the synthetic chemicals. Recently, the use of
biological pest control methods has become the most preferred method of controlling crop insect
pests. Several studies have been conducted on the use entomopathogenic nematodes on several
crops such as maize but intense research has not been conducted on the use of entomopathogenic
nematodes in the control of banana weevil borers. The objective of this study will be determining
the efficacy of entomopathogenic nematodes in control of banana weevil borers compared to
chemical control in banana plants. The experiment involved use of experimental units which
included metarhizium 69(EPNs in full) and karate 2.5(synthetic chemical treatment).The
experiment was laid in a completely randomized design with three treatments that is; EPN
treatment, chemical treatment and a control experiment replicated thrice. Adults of C. sordidus
adults were collected with pseudostem traps (roof tile-type baits) installed in the University of
Embu demo farm on nanicão banana cultivar. The insects were transferred to the laboratory,
kept in plastic containers with moistened sand and bits of banana pseudostem and kept at 26 ± 1º
C and natural photoperiod, where they remained until their use in experiment. Both chemical and
EPN treatments were applied at a rate of 1g per container. Data was be collected at an interval of
24 hours, 48 hours,7 days,14 days and finally 28 days. The data collected included mortality rate
of the weevils, number of weevils not affected by the treatment. The data collected was subjected
to analysis of variance (ANOVA) and differences among treatments compared using Tukey
mean separation test (p < 0.05) using the statistical program Sisvar. Results of the study showed
that all the treatments (Chemicals, EPNs and Control experiment) showed variability in the total
number of weevils present after every other day per replicate. Nematode numbers dropped as the
number of days increased and plots treated with entomopathogenic nematodes had the least
number of nematodes. It is recommended that the study be repeated for another season to
confirm the results of this study.
