A method to synthesize cDNA constructs by homology based recombination cloning

dc.contributor.author	Verma, Neetu
dc.contributor.author	Burma, Pradeep K.
dc.date.accessioned	2016-10-17T08:17:00Z
dc.date.available	2016-10-17T08:17:00Z
dc.date.issued	2014-01
dc.identifier.citation	American Journal of Molecular Biology, 2014, 4, 16-19	en_US
dc.identifier.uri	http://dx.doi.org/10.4236/ajmb.2014.41003
dc.identifier.uri	http://hdl.handle.net/123456789/958
dc.description.abstract	We introduce a homology-based recombination approach for generating a cDNA construct. This method depends on amplifying several exon fragments and their fusions by the homology-based recombination. This method provides a way to generate the cDNA sequence of any gene without any need for its mRNA. The paper describes the strategy by assembling cDNA of the MYB1 and MYB2 genes of Arabidopsis thaliana.	en_US
dc.language.iso	en	en_US
dc.publisher	Scientific Research Publishing	en_US
dc.subject	Homology Based Recombination Cloning	en_US
dc.subject	cDNA Assembly	en_US
dc.title	A method to synthesize cDNA constructs by homology based recombination cloning	en_US
dc.type	Article	en_US

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