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Inteins—A Focus on the Biotechnological Applications of Splicing-Promoting Proteins

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dc.contributor.author Miraula, Manfredi
dc.contributor.author Enculescu, Charmaine
dc.contributor.author Mitić, Natasa
dc.contributor.author Schenk, Gerhard
dc.date.accessioned 2016-06-20T06:27:30Z
dc.date.available 2016-06-20T06:27:30Z
dc.date.issued 2015-03
dc.identifier.citation American Journal of Molecular Biology, 2015, 5, 42-56 en_US
dc.identifier.uri http://dx.doi.org/10.4236/ajmb.2015.52005
dc.identifier.uri http://hdl.handle.net/123456789/826
dc.description.abstract The main aim of this mini-review is to illustrate strategies and industrial applications based on inteins (INTErnal proteINS), which belong to a class of autocatalytic enzymes that are able to perform a catalytic reaction on a single substrate. However, since practical applications of inteins are strongly guided by a detailed understanding of their biological mechanisms and functions, the first part of this review will thus briefly discuss the physiological roles of inteins, describing what is currently known about their mechanisms of action. In the second part, specific biotechnological applications of inteins will be outlined (i.e. their use for (i) the purification of recombinant proteins, (ii) the cyclization of proteins and (iii) the production of seleno-proteins), paying attention to both potential strengths and weaknesses of this technology. en_US
dc.language.iso en en_US
dc.publisher Scientific Research Publishing en_US
dc.subject Intein en_US
dc.subject Protein Purification en_US
dc.subject Tagged Protein en_US
dc.subject Cyclization en_US
dc.subject Selenoprotein en_US
dc.title Inteins—A Focus on the Biotechnological Applications of Splicing-Promoting Proteins en_US
dc.type Article en_US


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