Biology and Biotechnology: Recent submissions

Now showing items 101-120 of 193

Characterization of winged bean (Psophocarpus tetragonolobus (L.) DC.) based on molecular, chemical and physiological parameters

Mohanty, Chandra S.; Verma, Sushma; Singh, Vinayak; Khan, Shahina; Gaur, Priyanka; Gupta, Priya; Nizar, M. Abdul; Dikshit, Nilamani; Pattanayak, Rojalin; Shukla, Alpika; Niranjan, Abhishekh; Sahu, Nayan; Behera, Soumit K.; Rana, Tikam S. (Scientific Research Publishing, 2013-10)

Winged bean (Psophocarpus tetragonolobus (L.) DC.) is a potential legume crop of the tropics with high protein and oil content in the seeds. Analysis of the mutual genotypic relationships among twenty four genotypes of P. ...
Identification and preliminary characterization of novel B3-type metallo-β-lactamases

Miraula, Manfredi; Brunton, Conor S.; Schenk, Gerhard; Mitić, Nataša (Scientific Research Publishing, 2013-10)

Antibiotic resistance has emerged as a major global threat to human health. Among the strategies employed by pathogens to acquire resistance the use of metallo-β-lactamases (MBLs), a family of dinuclear metalloenzymes, is ...
Interaction between the Wilms tumour factor-1 element in the promoter of Amh and a downstream enhancer is required for a strong expression of the gene in pre-pubertal sertoli cells

Dresser, David W. (Scientific Research Publishing, 2013-07)

Amh (anti-Müllerian hormone) is a single copy gene which is expressed strongly in Sertoli cells in the foetal testis and participates in the onset of sexual differentiation. Its promoter driving the expression of a reporter ...
Determination and inheritance of phytic acid as marker in diverse genetic group of bread wheat

Ahmad, Ijaz; Mohammad, Fida; Zeb, Aurang; Noorka, Ijaz R.; Farhatullah; Jadoon, Sultan A. (Scientific Research Publishing, 2013-07)

Phytic acid (Myo-inositol 1,2,3,4,5,6 hexa-kisphophate) is a storage form of phosphorus and can accumulate to the levels as high as 35% in the wheat kernel. Phytic acid acts as an inhibitor for macronutrients as well as ...
Genetic diversity of hybrid durian resulted from cross breeding between Durio kutejensis and Durio zibethinus based on random amplified polymorphic DNAs (RAPDs)

Hariyati, Tati; Kusnadi, Joni; Arumingtyas, Estri L. (Scientific Research Publishing, 2013-07)

One of the ways to improve the quality of Indonesian Durian is by utilizing germplasm diversity. Durio zibethinus is the most cultivated durian in Indonesia, whereas Durio kutejensis is a unique durian cultivar which has ...
Quantitative detection of Streptococcus mutans from saliva using FTATM elute cards and real-time polymerase chain reaction

Seghatoleslami, Sepideh; Ohlsson, Lars; Hamberg, Kristina; Carlsson, Peter; Ericson, Dan; Ljunggren, Lennart (Scientific Research Publishing, 2013-07)

Dental caries is a localized, transmissible, pathological infection process that ends up in the destruction of hard dental tissue. Numerous reports have shown the close relationship between salivary levels of Streptococcus ...
Generation of pig primary fibroblast cells harboring defective MC4R genes by N-ethyl-N-nitrosourea mutagenesis: A gene-driven, nontransgenic approach to pig improvement

Sakurai, Michiharu; Suzuki, Shun-ichi; Furusawa, Tadashi; Mikawa, Satoshi; Tokunaga, Tomoyuki; Onishi, Akira; Awata, Takashi (Scientific Research Publishing, 2013-06)

Transgenic pigs have been produced with the aim of further improving pigs in terms of economic and environmental traits, but these animals have not been allowed to enter the food chain. As an alternative approach to ...
Genome sequencing and next-generation sequence data analysis: A comprehensive compilation of bioinformatics tools and databases

Jimenez-Lopez, Jose C.; Gachomo, Emma W.; Sharma, Sweta; Kotchoni, Simeon O. (Scientific Research Publishing, 2013-04)

Genomics has become a ground-breaking field in all areas of the life sciences. The advanced genomics and the development of high-throughput techniques have lately provided insight into whole-genome characterization of a ...
Research of total levels on DNA methylation in plant based on HPLC analysis

Chen, Qiang; Tao, Siyuan; Bi, Xiaohua; Xu, Xin; Wang, Lanlan; Li, Xuemei (Scientific Research Publishing, 2013-04)

HPLC analysis is important for determination of total level on DNA methylation in plants. It can be used to help characterise epigenetic changes during growth, development and stress. HPLC methods have been optimised for ...
1,25-Dihydroxyvitamin D3 effects on the regulation of the insulin receptor gene in the hind limb muscle and heart of streptozotocin-induced diabetic rats

Calle, Consuelo; Maestro, Begoña; García-Arencibia, Moisés (Scientific Research Publishing, 2013-04)

In the present study, we examine the effects of the treatment with 1,25-dihydroxyvitamin D3 [150 IU/Kg (3.75 μg/Kg) once a day, for 15 days] to non-diabetic and streptozotocin-induced diabetic rats. The results indicate ...
Breeding of NHase hyper-producing Rhodococcus ruber strain LUV30-06 and verification of mutants by RAPD

Wang, Shiwei; Wang, Min; Wang, Qinghui (Scientific Research Publishing, 2013-04)

A nitrile hydratase (NHase) hyper-producing Rhodococcus ruber strain LUV30-06 was bred by mutagenization on the starting strain CGMCC3090 with ultraviolet irradiation and lithium chloride. The NHase activity of the strain ...
Plasmid instability when the hsp60 gene promoter is used to express the protective non-toxic fragment B of the diphtheria toxin in recombinant BCG

Nascimento, Dilzamar V.; Dellagostin, Odir A.; Junior, Raphael H.; Pereira, Geraldo M.; Mattos-Guaraldi, Ana L.; Armôa, Geraldo R. (Scientific Research Publishing, 2013-04)

The genetic modification of the live attenuated Mycobacterium bovis BCG to deliver a protective Corynebacterium diphtheriae antigen in vivo could be a safer and less costly alternative to the new and more expensive DTP ...
Effect of SP600125 on proliferation of embryonic stem cell

Zhou, Yonghua; Jiang, Minggui; Wang, Mei; Luo, Chao; Wang, Zili; Wen, Sheng; Liu, Wenbin; Xiao, Yamei (Scientific Research Publishing, 2013-04)

SP600125 is an inhibitor of c-Jun NH2-terminal kinase (JNK), which plays a fundamental role in regulating animal development. Using SP600125 to deal with the mouse embryonic stem cells, it is revealed that the number of ...
The molecular regulatory effect of intracerebroventricular thymulin on endotoxin-mediated NF-kB nuclear translocation and activation in vivo

Haddad, John J. (Scientific Research Publishing, 2013-01)

The nuclear factor-kB (NF-kB) is one member of a ubiquitously expressed family of Rel-related transcription factors that serve as critical regulators of proinflammatory genes. The immunomodulatory potential of thymulin and ...
Characterization of the role of Smu1 in nuclear localization of splicing factors in the mammalian temperature-sensitive mutant

Sugaya, Kimihiko; Ishihara, Yoshie; Sugaya, Keiko; Inoue, Sonoe (Scientific Research Publishing, 2013-01)

A temperature-sensitive (ts) mutant of the CHO-K1 cell line, tsTM18, grows at 340C but not at 390C. Smu1 is the gene responsible for ts defects of tsTM18 cells. Previously, we found that the Smu1 ts defect altered the ...
Identification and characterization drought tolerance of gene LEA-D11 soybean (glycine max L. Merr) based on PCR-sequencing

Savitri, Evika S.; Basuki, Nur; Aini, Nurul; Arumingtyas, Estri L. (Scientific Research Publishing, 2013-01)

Drought is one of the most damaging abiotic stress. Different plants response differently to drought stress. Abiotic stresses such as drought induced diverse physicological and molecular responses in plants. These responses ...
Using Bayesian and Eigen approaches to study spatial genetic structure of Moroccan and Syrian durum wheat landraces

Kehel, Zakaria; Garcia-Ferrer, Alfonso; Nachit, Miloudi M. (Scientific Research Publishing, 2013-01)

The Mediterranean durum wheat landraces are genetically diverse and important sources for improving resistance to abiotic and biotic stresses and developing adapted and productive durum wheat varieties in the Mediterranean ...
Frequently cutting restriction enzymes: Clearing the fog to see the ends

Vaquero-Sedas, María I.; Vega-Palas, Miguel A. (Scientific Research Publishing, 2013-01)

Telomeres, which are found at the ends of eukaryotic chromosomes, are composed of tandem arrays of repetitive sequences and safeguard genomic stability. Previous studies have revealed that telomeric repeats are also present ...
Bax promoter G(－248)A polymorphism in a Turkish clinical breast cancer patients: A case-control study

Yildiz, Yemliha; Yaylim, Ilhan; Ozkan, Nazli E.; Arikan, Soykan; Turan, Saime; Kucucuk, Seden; Coskunpinar, Ender; Isbir, Turgay (Scientific Research Publishing, 2013-01)

Bax is an important protein involved in apoptotic process. Mutations of the Bax gene are known to af- fect protein expression and function. A promotor polymorphism G(－248)A in the 5’UTR of Bax gene may alter regulation of ...
Effect of 4% glycerol and low aeration on result of expression in Escherichia coli of Cin3 and three Venturia inaequalis EST’s recombinant proteins

Al-Samarrai, Taha H.; T. Jones, William; Harvey, Dawn; A. Kirk, Christopher; Templtone, M. (Scientific Research Publishing, 2013-01)

The phytopathogenic fungus Venturia inaequalis causes scab of apple. Once this fungus penetrates the plant surface, it forms a specialized body called a stroma between the inner cuticle surface and the epidermal cell wall. ...

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