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Development of cost-effective media for the culture of Chilo partellus larvicide in Kenya

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dc.contributor.author Anyika, D.
dc.contributor.author Boga, Hamadi I.
dc.contributor.author Mwirichia, Romano K.
dc.date.accessioned 2015-07-23T16:21:37Z
dc.date.available 2015-07-23T16:21:37Z
dc.date.issued 2013-03
dc.identifier.citation International Journal for Biotechnology and Molecular Biology Research Vol. (1), pp. 1-8 en_US
dc.identifier.issn 2141-2154
dc.identifier.uri http://hdl.handle.net/123456789/307
dc.description DOI: 10.5897/IJBMBR12.011 en_US
dc.description.abstract Stem borers (Chilo partellus) are important field insect pests of maize (Zea mays L.) and sorghum (Sorghum bicolor L.) in Africa. They account for more than 30% yield losses depending on the composition of the pest community. C. partellus larvicide like Bacillus thuringiensis have been widely and effectively used in C. partellus control programs, but the industrial production of theses bacilli is expensive. Here we have attempted to develop three cost-effective media, based on legumes, potato, and whey. Growth and production of the insecticidal proteins from these bacteria were satisfactory; protein concentration yields of 27.60 mg/ml, spore counts of 5.60 × 108 CFU/ ml and first-instar Chilo partellus larvicidal activity (LC50) of 78 μg/l were obtained with a 72 h culture of this bacterium. Therefore, this investigation suggests that legume, potato and whey-based culture media are more economical and effective for the industrial production of B. thuringiensis insecticidal crystal proteins. en_US
dc.language.iso en en_US
dc.publisher Academic Journals en_US
dc.subject Bacillus thuringiensis en_US
dc.subject larvicidal en_US
dc.subject Chilo partellus en_US
dc.subject insecticidal crystal proteins en_US
dc.subject LC50 en_US
dc.subject optical density en_US
dc.subject sulphate polyacrylamide gel electrophoresis (SDS-PAGE) en_US
dc.subject spore counts en_US
dc.title Development of cost-effective media for the culture of Chilo partellus larvicide in Kenya en_US
dc.type Article en_US


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