Purification of Dengue Virus Particles by One-Step Ceramic Hydroxyapatite Chromatography

Kurosawa, Yae; Saito, Maiko; Kobayashi, Shintaro; Okuyama, Tsuneo

dc.contributor.author	Kurosawa, Yae
dc.contributor.author	Saito, Maiko
dc.contributor.author	Kobayashi, Shintaro
dc.contributor.author	Okuyama, Tsuneo
dc.date.accessioned	2018-06-25T06:06:14Z
dc.date.available	2018-06-25T06:06:14Z
dc.date.issued	2012-08
dc.identifier.citation	World Journal of Vaccines, 2012, 2, 155-160	en_US
dc.identifier.uri	http://dx.doi.org/10.4236/wjv.2012.23020
dc.identifier.uri	http://hdl.handle.net/123456789/1535
dc.description.abstract	Dengue virus type 2 ThNH7/93 retained infectious activity after purification by ceramic hydroxyapatite chromatography. Dengue virus type 2 culture fluid was loaded onto the ceramic hydroxyapatite column and eluted with a linear gradient of sodium phosphate buffer. Culture fluid and protein contaminants derived from host cells were eluted initially, followed by elutions of dsDNA, and then dengue virus type 2. The recoveries of dengue virus type 2 were 64 ± 14% (n = 11) in the hemagglutination (HA) test and 60% (n = 2) determined by focus assay for viral infectivity. This protocol was highly reproducible, simple, rapid, and appears applicable to other virus species such as influenza virus, Japanese encephalitis virus and adenovirus	en_US
dc.description.sponsorship	Department of Virology, Institute of Tropical Medicine, Nagasaki University, Japan. Department of Virology, National Institute of Infectious Diseases, Japan.	en_US
dc.language.iso	en	en_US
dc.publisher	Scientific Research	en_US
dc.subject	Ceramic Hydroxyapatite	en_US
dc.subject	Virus Purification	en_US
dc.subject	Dengue Virus	en_US
dc.subject	Virus Particle	en_US
dc.subject	Virion	en_US
dc.title	Purification of Dengue Virus Particles by One-Step Ceramic Hydroxyapatite Chromatography	en_US
dc.type	Article	en_US