Breeding of NHase hyper-producing Rhodococcus ruber strain LUV30-06 and verification of mutants by RAPD

dc.contributor.author	Wang, Shiwei
dc.contributor.author	Wang, Min
dc.contributor.author	Wang, Qinghui
dc.date.accessioned	2016-10-25T14:15:35Z
dc.date.available	2016-10-25T14:15:35Z
dc.date.issued	2013-04
dc.identifier.citation	American Journal of Molecular Biology, 2013, 3, 108-114	en_US
dc.identifier.uri	http://dx.doi.org/10.4236/ajmb.2013.32015
dc.identifier.uri	http://hdl.handle.net/123456789/1036
dc.description.abstract	A nitrile hydratase (NHase) hyper-producing Rhodococcus ruber strain LUV30-06 was bred by mutagenization on the starting strain CGMCC3090 with ultraviolet irradiation and lithium chloride. The NHase activity of the strain LUV30-06 was increased by 21.99% (3881.3 U/ml), as compared with that of R. ruber CGMCC3090 (3181.4 U/ml). The mutant strain UV30-06 has been proved genetically stable with higher NHase activity in seven successive subcultures as well as random amplified polymorphic DNA analysis (RAPD).	en_US
dc.language.iso	en	en_US
dc.publisher	Scientific Research Publishing	en_US
dc.subject	Rhodococcus ruber	en_US
dc.subject	NHase	en_US
dc.subject	Mutagenesis	en_US
dc.subject	Random Amplified Polymorphic DNA	en_US
dc.title	Breeding of NHase hyper-producing Rhodococcus ruber strain LUV30-06 and verification of mutants by RAPD	en_US
dc.type	Article	en_US

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